Western Blot:Article Title: The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis
Article Snippet: .. Application rabbit anti-LBH Abcam, ab173737 WB (1:100) rabbit anti-LBH Abcam, ab122223 IF/IHF (1:100) rabbit anti-CRYAB Proteintech, 15808-1 WB (1:500) mouse anti-CRYAB Abcam, ab76467 IF/IHF (1:100); WB (1:1000) rabbit antiphospho-CRYAB Abcam, ab5577 WB (1:2000); IP (1:50); IHF (1:400) rabbit anti-GPX4 Cell Signaling Technology, 59735 WB (1:800) rabbit anti-NRF2 Proteintech, 16396-1 IF/IHF (1:400); WB (1:800) rabbit anti-KEAP1 Proteintech, 10503-2 WB (1:800) rabbit anti-Lamin B1 Proteintech, 12987-1 WB (1:800) mouse anti-p53 Abcam, ab26 IF/IHF (1:200); IP (1:50) rabbit anti-p53 Proteintech, 10442-1 WB (1:800) rabbit anticleaved Caspase 3 Cell Signaling Technology, 9661S WB (1:800) rabbit anti-Bax Sigma, B3428 WB (1:800) rabbit anti-BCL2 Sigma, SAB4500003 WB (1:800) rabbit anti-HIF-1α Thermo Scientific, PA1-16601 WB (1:1000) rabbit anti-p38 Cell Signaling Technology, 9212S WB (1:800) rabbit anti-phospho-p38 Cell Signaling Technology, 9211S WB (1:800) rabbit anti-GAPDH Bioworlde, AP0063 WB (1:8000) mouse anti-α-Tubulin Proteintech, 66031-1 WB (1:2000) mouse anti-β-Actin Bioworlde, BS6007M WB (1:5000) rabbit anti-Cardiac myosin Abcam, ab46936 IF (1:2000) rabbit anti-CTnT Abcam, EPR20266 IF (1:2000) rabbit anti-α-Actinin Abcam, ab68194 IF (1:1000) rabbit anti-GSDMD Novus, NBP2-33422 WB (1:800) rabbit anti-phospho-MLKL Novus, NBP2-66953 WB (1:800) rabbit anti-LC3 Abcam, ab192890 WB (1:800) goat anti-rabbit IgG-HRP Cell Signaling Technology, 7074 WB (1:4000) horse anti-mouse IgG-HRP Cell Signaling Technology, 7076 WB (1:4000) goat anti-mouseAlexa Fluor 488 Invitrogen A-11001 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 488 Invitrogen A-21206 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 555 Invitrogen A-31572 IF (1:500) goat anti-mouseAlexa Fluor 790 Invitrogen A-11357 IHF (1:500) goat anti-rabbitAlexa Fluor 680 Invitrogen A-32734 IHF (1:500) anti-mouse IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 001-100-005 WB (1:2000) anti-rabbit IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 025-100-005 WB (1:2000) WB, western blotting; IF, Immunofluorescence; IHF, Immunohistofluorescence; IP, Coimmunoprecipitation. ..
Immunohistofluorescence:Article Title: The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis
Article Snippet: .. Application rabbit anti-LBH Abcam, ab173737 WB (1:100) rabbit anti-LBH Abcam, ab122223 IF/IHF (1:100) rabbit anti-CRYAB Proteintech, 15808-1 WB (1:500) mouse anti-CRYAB Abcam, ab76467 IF/IHF (1:100); WB (1:1000) rabbit antiphospho-CRYAB Abcam, ab5577 WB (1:2000); IP (1:50); IHF (1:400) rabbit anti-GPX4 Cell Signaling Technology, 59735 WB (1:800) rabbit anti-NRF2 Proteintech, 16396-1 IF/IHF (1:400); WB (1:800) rabbit anti-KEAP1 Proteintech, 10503-2 WB (1:800) rabbit anti-Lamin B1 Proteintech, 12987-1 WB (1:800) mouse anti-p53 Abcam, ab26 IF/IHF (1:200); IP (1:50) rabbit anti-p53 Proteintech, 10442-1 WB (1:800) rabbit anticleaved Caspase 3 Cell Signaling Technology, 9661S WB (1:800) rabbit anti-Bax Sigma, B3428 WB (1:800) rabbit anti-BCL2 Sigma, SAB4500003 WB (1:800) rabbit anti-HIF-1α Thermo Scientific, PA1-16601 WB (1:1000) rabbit anti-p38 Cell Signaling Technology, 9212S WB (1:800) rabbit anti-phospho-p38 Cell Signaling Technology, 9211S WB (1:800) rabbit anti-GAPDH Bioworlde, AP0063 WB (1:8000) mouse anti-α-Tubulin Proteintech, 66031-1 WB (1:2000) mouse anti-β-Actin Bioworlde, BS6007M WB (1:5000) rabbit anti-Cardiac myosin Abcam, ab46936 IF (1:2000) rabbit anti-CTnT Abcam, EPR20266 IF (1:2000) rabbit anti-α-Actinin Abcam, ab68194 IF (1:1000) rabbit anti-GSDMD Novus, NBP2-33422 WB (1:800) rabbit anti-phospho-MLKL Novus, NBP2-66953 WB (1:800) rabbit anti-LC3 Abcam, ab192890 WB (1:800) goat anti-rabbit IgG-HRP Cell Signaling Technology, 7074 WB (1:4000) horse anti-mouse IgG-HRP Cell Signaling Technology, 7076 WB (1:4000) goat anti-mouseAlexa Fluor 488 Invitrogen A-11001 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 488 Invitrogen A-21206 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 555 Invitrogen A-31572 IF (1:500) goat anti-mouseAlexa Fluor 790 Invitrogen A-11357 IHF (1:500) goat anti-rabbitAlexa Fluor 680 Invitrogen A-32734 IHF (1:500) anti-mouse IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 001-100-005 WB (1:2000) anti-rabbit IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 025-100-005 WB (1:2000) WB, western blotting; IF, Immunofluorescence; IHF, Immunohistofluorescence; IP, Coimmunoprecipitation. ..
Immunofluorescence:Article Title: The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis
Article Snippet: .. Application rabbit anti-LBH Abcam, ab173737 WB (1:100) rabbit anti-LBH Abcam, ab122223 IF/IHF (1:100) rabbit anti-CRYAB Proteintech, 15808-1 WB (1:500) mouse anti-CRYAB Abcam, ab76467 IF/IHF (1:100); WB (1:1000) rabbit antiphospho-CRYAB Abcam, ab5577 WB (1:2000); IP (1:50); IHF (1:400) rabbit anti-GPX4 Cell Signaling Technology, 59735 WB (1:800) rabbit anti-NRF2 Proteintech, 16396-1 IF/IHF (1:400); WB (1:800) rabbit anti-KEAP1 Proteintech, 10503-2 WB (1:800) rabbit anti-Lamin B1 Proteintech, 12987-1 WB (1:800) mouse anti-p53 Abcam, ab26 IF/IHF (1:200); IP (1:50) rabbit anti-p53 Proteintech, 10442-1 WB (1:800) rabbit anticleaved Caspase 3 Cell Signaling Technology, 9661S WB (1:800) rabbit anti-Bax Sigma, B3428 WB (1:800) rabbit anti-BCL2 Sigma, SAB4500003 WB (1:800) rabbit anti-HIF-1α Thermo Scientific, PA1-16601 WB (1:1000) rabbit anti-p38 Cell Signaling Technology, 9212S WB (1:800) rabbit anti-phospho-p38 Cell Signaling Technology, 9211S WB (1:800) rabbit anti-GAPDH Bioworlde, AP0063 WB (1:8000) mouse anti-α-Tubulin Proteintech, 66031-1 WB (1:2000) mouse anti-β-Actin Bioworlde, BS6007M WB (1:5000) rabbit anti-Cardiac myosin Abcam, ab46936 IF (1:2000) rabbit anti-CTnT Abcam, EPR20266 IF (1:2000) rabbit anti-α-Actinin Abcam, ab68194 IF (1:1000) rabbit anti-GSDMD Novus, NBP2-33422 WB (1:800) rabbit anti-phospho-MLKL Novus, NBP2-66953 WB (1:800) rabbit anti-LC3 Abcam, ab192890 WB (1:800) goat anti-rabbit IgG-HRP Cell Signaling Technology, 7074 WB (1:4000) horse anti-mouse IgG-HRP Cell Signaling Technology, 7076 WB (1:4000) goat anti-mouseAlexa Fluor 488 Invitrogen A-11001 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 488 Invitrogen A-21206 IF (1:500) donkey anti-rabbitAlexa Fluor Plus 555 Invitrogen A-31572 IF (1:500) goat anti-mouseAlexa Fluor 790 Invitrogen A-11357 IHF (1:500) goat anti-rabbitAlexa Fluor 680 Invitrogen A-32734 IHF (1:500) anti-mouse IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 001-100-005 WB (1:2000) anti-rabbit IgG for IP, AlpsdAbs@VHH (HRP) AlpVHHs, 025-100-005 WB (1:2000) WB, western blotting; IF, Immunofluorescence; IHF, Immunohistofluorescence; IP, Coimmunoprecipitation. ..
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